Bone erosion is a pathological condition characterized by breaks in the cortical bone surface and loss of the adjacent trabecular bone. Several pathological processes can lead to bone erosion, including malignant tumors, abnormal metabolic processes such as hyperparathyroidism, and chronic inflammatory diseases such as rheumatoid arthritis. In clinical settings, bone erosion is routinely detected using X-ray based imaging technologies such as computed tomography (CT). Although preclinical CT offers high resolution 3D imaging for bone, accessibility to this modality may be challenging. Learn how optical and high-resolution X-ray imaging capability on the IVIS Lumina X5 system and Living Image® software was used for obtaining high quality images for quantitative analysis in a mouse bone erosion model, with the ease and speed of 2D imaging.